EGFR ET1604-44 信号传导 &酪氨酸蛋白激酶
左侧为HUABIO 右侧为竞品
1:500 稀释条件下， HUABIO (ET1604-44 ) ICC 应用可以替代竞品
Immunocytochemistry analysis of A431 cells labeling EGFR with Rabbit anti-EGFR antibody (ET1604-44) at 1/500 dilution and competitor's antibody at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody (ET1604-44) at 1/500 dilution and competitor's antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
华安生物信号传导推荐产品：
1. GLUT1 货号：ET1601-10
Anti-Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]
2. gamma Adaptin 货号：HA721843
Anti-gamma Adaptin Recombinant Rabbit Monoclonal Antibody [PSH02-65]
3. GPX4 货号：ET1706-45
Anti-GPX4 Recombinant Rabbit Monoclonal Antibody [JU11-31]
4. INPP4A 货号：HA721651
Anti-INPP4A Recombinant Rabbit Monoclonal Antibody [PSH01-27]
左侧为HUABIO 右侧为竞品
1:500 稀释条件下， HUABIO (ET1604-44 ) ICC 应用可以替代竞品
Immunocytochemistry analysis of A431 cells labeling EGFR with Rabbit anti-EGFR antibody (ET1604-44) at 1/500 dilution and competitor's antibody at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody (ET1604-44) at 1/500 dilution and competitor's antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
华安生物信号传导推荐产品：
1. GLUT1 货号：ET1601-10
Anti-Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]
2. gamma Adaptin 货号：HA721843
Anti-gamma Adaptin Recombinant Rabbit Monoclonal Antibody [PSH02-65]
3. GPX4 货号：ET1706-45
Anti-GPX4 Recombinant Rabbit Monoclonal Antibody [JU11-31]
4. INPP4A 货号：HA721651
Anti-INPP4A Recombinant Rabbit Monoclonal Antibody [PSH01-27]
