研究报告：研究了选自由磷脂酰胆碱，胆固醇和联十六烷基磷酸盐 的磷脂囊泡的相互作用 胎牛血清 和血清蛋白的级分的作用（摩尔比7：2：1），其中在一个主单层培养大鼠肝实质细胞。
这样囊泡与标记的磷脂酰胆碱的胎牛血清部分的温育过程中被转移到类似于我们先前鉴定为高密度脂蛋白（Scherphof，G，Roerdink，FH，韦特，M。和公园的衍生物的一个脂蛋白粒子，J。（1978）生物化学生物生物物理学报542，296-307）。当如此形成的粒子与细胞一起孵育时观察到的磷脂标签的转移到细胞中。
当囊泡与血清这种脂蛋白介导的脂质转移的存在下将细胞温育可以设想有助于所观察到的总脂质摄取。然而，我们发现，胎牛血清的培养基中的存在大大降低，而不是提高脂质体的脂质的总转移到细胞中。还牛血清白蛋白和牛β球蛋白减少这种转变中，虽然比全血清程度较轻。α-球蛋白，另一方面，分别为在降低脂质体磷脂的摄入完整血清一样有效。一个γ球蛋白馏分未能显示出在[14 C]胆碱，由细胞摄取的任何影响。
这能够抑制脂质体磷脂的细胞摄取所有蛋白质的级分显示出结合到磷脂囊泡。此外，脂质囊泡预温育有胎牛血清，然后从中分离出显示减少标记的磷脂酰胆碱转移至实质细胞。
这些观察被带到表明脂质体的脂质的减弱摄取可以通过如结合某些血清蛋白的结果的脂质体表面的膜的变形而引起。
英文原文：
We studied the effect of fetal calf serum and serum protein fractions on the interaction of phospholipid vesicles consisting of phosphatidylcholine, cholesterol and dicetylphosphate (molar ratio 7 : 2 : 1), with rat liver parenchymal cells in a primary monolayer culture. During incubation of such vesicles with fetal calf serum part of the labeled phosphatidylcholine is transferred to a lipoprotein particle similar to the one we identified previously as a derivative of high density lipoprotein (Scherphof, G., Roerdink, F.H., Waite, M. and Parks, J. (1978) Biochim. Biophys. Acta 542, 296–307). When the particle thus formed is incubated with the cells a transfer of the phospholipid label to the cells is observed. When vesicles are incubated with the cells in presence of serum such lipoprotein-mediated lipid transfer may conceivably contribute to the total lipid uptake observed.
However, we found that the presence of fetal calf serum in the culture medium greatly diminished rather than increased the total transfer of liposomal lipid to the cells. Also bovine serum albumin and bovine β-globulins reduced this transfer, although to a lesser extent than whole serum. α-Globulins, on the other hand, were as effective as complete serum in reducing the uptake of liposomal phospholipid. A γ-globulin fraction failed to exhibit any effect on the uptake of [14C]phosphatidylcholine by the cells.
All protein fractions which were able to inhibit cellular uptake of liposomal phospholipid were shown to bind to the phospholipid vesicles. Furthermore, lipid vesicles preincubated with fetal calf serum and then separated from it showed reduced transfer of labeled phosphatidylcholine to parenchymal cells.
These observations were taken to suggest that the diminished uptake of liposomal lipid may be caused by a modification of the liposomal surface membrane as a result of the binding of certain serum proteins.
目前广泛应用于细胞培养的是胎牛血清，可用于：病毒载体生产、细胞治疗、哺乳动物细胞培养。胎牛血清是生物制品生产中不可或缺的一种原材料，它的品质会直接影响到生物制品的质量及安全性。虽然减少动物来源成分是现在生物制品行业发展的趋势，可是离攻克无血清的技术还有很长一段距离，胎牛血清依旧在细胞培养领域中占据非常重要的地位。如：Ausbian品牌胎牛血清。好的实验结果离不开优质的实验材料，那好的胎牛血清应具备什么特点呢？
1. 精选极低内毒素低，内毒素选取小于3Eu/ml，可以使细胞更健康。
如果选取内毒素较高的血清，细胞一直泡在毒液里，短期内试不出结果，用不健康的细胞做实验，对实验结果造成严重影响。
2. 同批次数量多，保证了实验更加顺利的同时，节省了试用的时间和成本。
3.具有详细完整的《检测报告》，包括：品名，货号，批号，血源地，生产日期，保质期，pH值、渗透压、血红蛋白、总蛋白、球蛋白、IgG、内毒素、无菌检查（细菌、真菌、支原体）、病毒检查（如BVD、牛腺病毒、细胞病变效应等）、促细胞生长能力、细胞毒性、BVD-1/2抗体检查等。
实验人对于新批次血清，应认真审阅《检测报告》，做好试用前筛选第一步。
这样囊泡与标记的磷脂酰胆碱的胎牛血清部分的温育过程中被转移到类似于我们先前鉴定为高密度脂蛋白（Scherphof，G，Roerdink，FH，韦特，M。和公园的衍生物的一个脂蛋白粒子，J。（1978）生物化学生物生物物理学报542，296-307）。当如此形成的粒子与细胞一起孵育时观察到的磷脂标签的转移到细胞中。
当囊泡与血清这种脂蛋白介导的脂质转移的存在下将细胞温育可以设想有助于所观察到的总脂质摄取。然而，我们发现，胎牛血清的培养基中的存在大大降低，而不是提高脂质体的脂质的总转移到细胞中。还牛血清白蛋白和牛β球蛋白减少这种转变中，虽然比全血清程度较轻。α-球蛋白，另一方面，分别为在降低脂质体磷脂的摄入完整血清一样有效。一个γ球蛋白馏分未能显示出在[14 C]胆碱，由细胞摄取的任何影响。
这能够抑制脂质体磷脂的细胞摄取所有蛋白质的级分显示出结合到磷脂囊泡。此外，脂质囊泡预温育有胎牛血清，然后从中分离出显示减少标记的磷脂酰胆碱转移至实质细胞。
这些观察被带到表明脂质体的脂质的减弱摄取可以通过如结合某些血清蛋白的结果的脂质体表面的膜的变形而引起。
英文原文：
We studied the effect of fetal calf serum and serum protein fractions on the interaction of phospholipid vesicles consisting of phosphatidylcholine, cholesterol and dicetylphosphate (molar ratio 7 : 2 : 1), with rat liver parenchymal cells in a primary monolayer culture. During incubation of such vesicles with fetal calf serum part of the labeled phosphatidylcholine is transferred to a lipoprotein particle similar to the one we identified previously as a derivative of high density lipoprotein (Scherphof, G., Roerdink, F.H., Waite, M. and Parks, J. (1978) Biochim. Biophys. Acta 542, 296–307). When the particle thus formed is incubated with the cells a transfer of the phospholipid label to the cells is observed. When vesicles are incubated with the cells in presence of serum such lipoprotein-mediated lipid transfer may conceivably contribute to the total lipid uptake observed.
However, we found that the presence of fetal calf serum in the culture medium greatly diminished rather than increased the total transfer of liposomal lipid to the cells. Also bovine serum albumin and bovine β-globulins reduced this transfer, although to a lesser extent than whole serum. α-Globulins, on the other hand, were as effective as complete serum in reducing the uptake of liposomal phospholipid. A γ-globulin fraction failed to exhibit any effect on the uptake of [14C]phosphatidylcholine by the cells.
All protein fractions which were able to inhibit cellular uptake of liposomal phospholipid were shown to bind to the phospholipid vesicles. Furthermore, lipid vesicles preincubated with fetal calf serum and then separated from it showed reduced transfer of labeled phosphatidylcholine to parenchymal cells.
These observations were taken to suggest that the diminished uptake of liposomal lipid may be caused by a modification of the liposomal surface membrane as a result of the binding of certain serum proteins.
目前广泛应用于细胞培养的是胎牛血清，可用于：病毒载体生产、细胞治疗、哺乳动物细胞培养。胎牛血清是生物制品生产中不可或缺的一种原材料，它的品质会直接影响到生物制品的质量及安全性。虽然减少动物来源成分是现在生物制品行业发展的趋势，可是离攻克无血清的技术还有很长一段距离，胎牛血清依旧在细胞培养领域中占据非常重要的地位。如：Ausbian品牌胎牛血清。好的实验结果离不开优质的实验材料，那好的胎牛血清应具备什么特点呢？
1. 精选极低内毒素低，内毒素选取小于3Eu/ml，可以使细胞更健康。
如果选取内毒素较高的血清，细胞一直泡在毒液里，短期内试不出结果，用不健康的细胞做实验，对实验结果造成严重影响。
2. 同批次数量多，保证了实验更加顺利的同时，节省了试用的时间和成本。
3.具有详细完整的《检测报告》，包括：品名，货号，批号，血源地，生产日期，保质期，pH值、渗透压、血红蛋白、总蛋白、球蛋白、IgG、内毒素、无菌检查（细菌、真菌、支原体）、病毒检查（如BVD、牛腺病毒、细胞病变效应等）、促细胞生长能力、细胞毒性、BVD-1/2抗体检查等。
实验人对于新批次血清，应认真审阅《检测报告》，做好试用前筛选第一步。
