藤壶胶组分的分离制备
研究SephadexG-25,G-50,G-100,G-200和G-200(超细)等型号的葡聚糖凝胶系统,对溶解在1.0%SDS-2Me水溶液中的网纹藤壶Balanusreticulatus固态胶蛋白组分的分离制备效果。结果表明,SephadexG-200和G-200(超细)两种葡聚糖凝胶层析柱(直径1.7cm,长30cm),用0.05mol·L-1Tris-HCl-1%SDS-2Me溶液洗脱时,均可将改蛋白组分按其分圞子量的大小进行分离,且以后者的分高效果更好。
Because of the possible application as an underwater adhesive and its importance in antifouling fields, great attention has been gived to the physicochemical properties and polymerization mechaniSΜs of barnacle cement. In this paper, the separation of barnacle cement components by the Sephadex G-25, G-50, G-100, G-200, as well as G-200 (superfine ) polydextrаn gels for preparative purposes was studied. 50 mg solified cement of Balanus reticulatus was dissolved in 5 ml 1%SDS-2Me solution, and a 17mm X 300mm
研究SephadexG-25,G-50,G-100,G-200和G-200(超细)等型号的葡聚糖凝胶系统,对溶解在1.0%SDS-2Me水溶液中的网纹藤壶Balanusreticulatus固态胶蛋白组分的分离制备效果。结果表明,SephadexG-200和G-200(超细)两种葡聚糖凝胶层析柱(直径1.7cm,长30cm),用0.05mol·L-1Tris-HCl-1%SDS-2Me溶液洗脱时,均可将改蛋白组分按其分圞子量的大小进行分离,且以后者的分高效果更好。
Because of the possible application as an underwater adhesive and its importance in antifouling fields, great attention has been gived to the physicochemical properties and polymerization mechaniSΜs of barnacle cement. In this paper, the separation of barnacle cement components by the Sephadex G-25, G-50, G-100, G-200, as well as G-200 (superfine ) polydextrаn gels for preparative purposes was studied. 50 mg solified cement of Balanus reticulatus was dissolved in 5 ml 1%SDS-2Me solution, and a 17mm X 300mm